Direct desaturation of eicosatrienoyl lecithin to arachidonoyl lecithin by rat liver microsomes.

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Direct desaturation of eicosatrienoyl lecithin to arachidonoyl lecithin by rat liver microsomes.

A microsomal enzyme system from rat liver was shown to catalyze desaturation, in presence of reduced pyridine nucleotides and oxygen, of 1-acyl-2-[14C]eicosatrienoyl-sn-glycero-3-phosphorylcholine to 1-acyl-2-[14C]arachidonoyl-sn-glycerophosphorylcholine. This desaturation was linear with time and proportional to microsomal protein concentration, and proceeded with no significant breakdown of t...

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The phospholipids, along with cholesterol, are now recognized as essential elements of tissues, while the characteristic constituents of the phospholipids are the fatty acids. In any study of the metabolic activity of tissues the nature and proportions of the various fatty acids in the phospholipids therefore become important and have been the subject of increasing interest and speculation. Bec...

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Lecithin Synthesis, Intracellular Transport, and Secretion in Rat Liver

Injection of choline-(3)H into choline-deficient rats resulted in an enhanced incorporation of the label into liver lecithin, as compared to the incorporation of label into liver lecithin of normal rats. The results obtained with the use of different lecithin precursors indicate that in the intact liver cell, both in vivo and in vitro, exchange of choline with phosphatidyl-choline is not signif...

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A reliable, relatively simple method for isolation and quantification of disaturated lecithins is described. In rabbit lung, 34% of the lecithins were disaturated, in alveolar macrophages, 19%. More than 95% of the fatty acids of the disaturated lecithins from lung and alveolar macrophages was palmitic. Hence, the disaturated lecithins from these sources were essentially all dipalmitoyl lecithi...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 1977

ISSN: 0021-9258

DOI: 10.1016/s0021-9258(17)32799-0